Yeast/Fungi Methods

Contents

Colony PCR - provides a basic overview of the use of colony PCR in detecting recombinative events, both homologous and non-homologous from suspended S. pombe colonies.
The Definitive Yeast Transformation Home Page - offers four yeast transformation protocols with references.
Fission Yeast Transformation Procedures - includes three methods for transforming S. pombe with recombinant DNA: electroporation, lithium acetate treatment, and protoplast fusion.
Gel-Purified, Agarased YACs for Yeast Transformation - a protocol for purifying yeast artificial chromosomes by gel electrophoresis and retrieving the resultant products. The products are suitable for use in transformation experiments.
Herskowitz Lab Protocols - provides a wide variety of Saccharomyces cerevisiae protocols and supporting information. Covers topics including transformation, PCR, mutagenesis, and biochemical assays.
Microsome Preparation - a simple method for the isolation and preservation of microsomes from S. cerevisiae.
Working With Fission Yeast - contains a simple and effective protocol for the preparation of S. pombe cells for flow cytometry.
Yeast Cell Cycle Flow Cytometry Protocol - a protocol for the preparation of S. pombe cells for flow cytometry.

How to Grow Morel Mushrooms - detailed instructions for growing morel mushrooms indoors.
Fungal Genetics Stock Center Methods Page - offers advice, and a number of protocols dealing with microbiological problems in fungal genetics. The protocols center around cross conditions, culture media, and handling technique for Neurospora and Aspergillus species. The methods are presented in essay format, so speed of reading is sacrificed in favor of a detailed background for each set of protocols.
A Short 'Shroom Primer - a brief overview of many of the most common methods of edible mushroom identification. This guide does not provide information about identification of specific fungal species, but rather describes a group of tools which contribute to the accuracy of mushroom identification. The page also discusses the environmental and legal impacts of mushroom collection.

Last updated: July 27, 1998
URL: http://biotech.icmb.utexas.edu/pages/science/proyeafun.html
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