Botanical Methods

Contents

Arabidopsis: The Compleat Guide - a rare example of a Web page worthy of its title. The guide covers a truly extensive range of techniques and methods related to the study of Arabidopsis thaliana.
Arabidopsis Lab Protocols - a variety of protocols for growth and inoculation, microscropy, in situ hybridization, and DNA preps of Arabidopsis.
Green Lab Protocol for Vacuum Infiltration Transformation of Arabidopsis - a procedure which uses vacuum treatment to facilitate infiltration of Arabidopsis by recombinant DNA bearing strains of Agrobacterium.

Cell Transformation in Tobacco Leaf Disks - provides a tutorial protocol for the transformation of eukaryotic plant cells via infection with Agrobacterium.
Soybean Protocols - includes micropropagation (through somatic cell embryogenesis), transformation by DNA coated microprojectiles, Agrobacterium-mediated transformation, and preparing liquid culture media.
Wheat Transformation Procedures - includes wheat tissue culture recipes, sterile dissection, culture, and transformation of wheat immature embryos, biolistic transformation, molecular characterization of transgenic plants, etc.

How to Collect Voucher Specimens - explains how to collect, preserve, and label plants and pollen for use as an indicator of where insects are likely to settle or forage. Although text-heavy, it provides a respectable overview on how to dry and press plants for identification purposes.

Artificial Vegetative Propagation - explains some general procedures for the artificial stimulation of vegetative propogation in many types of plants. Methods covered inlcude stem cutting, leaf cutting, simple layering, air layering, and tissue culture.
Culturing Plants From Embryonic Plant Tissue - a very simple teaching laboratory, targeting first-year undergraduate and high-school biology students.
Plant Tissue Culture - a concise overview of plant micropropagation for the novice. The document focuses on encouraging plant enthusiasts with little biological background to carry out their own experiments in tissue culture to reproduce their favorite plants for enjoyment or business.

Trees

Dendrome Laboratory Protocols - a good basic set of tools for molecular genome analysis with a special emphasis on application to trees, and organisms related to forest health.
How To Diagnose Black Walnut Damage - a diagnostic reference for the various ailments that might be afflicting your favorite black walnut tree. Each of the most common assaults on black walnut health is then linked to detailed descriptions of the problem with recommendations for alleviating it.

Last updated: July 27, 1998
URL: http://biotech.icmb.utexas.edu/pages/science/probot.html
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